Pharmacological Mechanism of the Non-hallucinogenic 5-HT2A Agonist Ariadne and Analogs

This review (2022) provides a summary of the in-cell, in-animal and available clinical data with the non-hallucinogenic phenylalkylamine analogue Ariadne, and proposes a hypothesis for its lack of hallucinogenic effects and the therapeutic potential of this compound.

Abstract

“Ariadne is a non-hallucinogenic analogue in the phenylalkylamine chemical class of psychedelics that is closely related to an established synthetic hallucinogen, 2,5-dimethoxy-4-methyl-amphetamine (DOM), differing only by one methylene group in the α-position to the amine. Ariadne has been tested in humans, including clinical trials at Bristol-Myers Company that indicate a lack of hallucinogenic effects and remarkable therapeutic effects, such as rapid remission of psychotic symptoms in schizophrenics, relaxation in catatonics, complete remission of symptoms in Parkinson’s disease (PD), and improved cognition in geriatric subjects. Despite these provocative clinical results, the compound has been abandoned as a drug candidate, and its molecular pharmacology remained unknown. Here, we report a detailed examination of the in vitro and in vivo pharmacology of Ariadne and its analogues, and propose a molecular hypothesis for the lack of hallucinogenic effects and the therapeutic potential of this compound class. We also provide a summary of previous clinical and preclinical results to contextualize the molecular signalling data. Our results show that Ariadne is a serotonin 5-HT2 receptor agonist, exhibits modest selectivity over 5-HT1 receptors, has no relevant activity at 5-HT4,5,7 and other aminergic receptors, and no substantial affinity at plasma membrane monoamine transporters. Compared to DOM, Ariadne shows lower signalling potency and efficacy in multiple signalling pathways examined (Gq, G11, and β-arrestin2) coupled to 5-HT2A receptors. We confirmed the shift in signalling for an α-propyl analogue and provide a molecular docking rationale for the progressive decrease in signalling potency with the growing length of the α-substituent. Ariadne versus DOM exhibits no apparent change in the relative preference between Gq/11 activation and β-arrestin2 recruitment; instead, there is a small but consistent drop in efficacy in these signalling channels. Ariadne acts as a 5-HT2A agonist in vivo in mice and shows markedly attenuated head twitch response (HTR) in comparison to its hallucinogenic analogues, consistent with previous studies in rabbits, cats, and dogs. Hence, we propose the lower 5-HT2A receptor signalling efficacy of this compound class as an explanatory model for the lack of hallucinogenic effects of Ariadne in humans and the dramatically attenuated hallucinosis-like effects in animals (5-HT2A signalling efficacy hypothesis). In terms of reverse translation of the noted clinical therapeutic effects, we used an auxilin knockout model of Parkinson’s disease where Ariadne rescued severe motor deficits in this mouse line, on par with the effects of l-DOPA, a notable finding considering Ariadne’s lack of activity at dopamine receptors and transporters. Ariadne emerges as a prototype of a new drug class, non-hallucinogenic 5-HT_2A agonists, with considerable therapeutic potential across psychiatric and neurological indications.”

Authors: Michael J. Cunningham, Hailey A. Bock, Inis C. Serrano, Benjamin Bechand, D. J. Vidyadhara, Emma M. Bonniwell, David Lankri, Priscilla Duggan, Antonina L. Nazarova, Andrew B. Cao, Maggie M. Calkins, Prashant Khirsariya, Christopher Hwu, Vsevolod Katritch, Sreeganga S. Chandra, John D. McCorvy & Dalibor Sames